Under general supervision of two PI's, the incumbent will be expected to make a significant research contribution to the laboratories by conducting complex studies using molecular and cellular methods. A primary focus will be on in vitro and in vivo models of viral infection. The incumbent will be responsible for performing assays to assess how genetic or pharmacological manipulations impact viral infection using both BSL3 live virus infection and BSL2 pseudovirus infection assays.
The incumbent will perform a wide variety of molecular biology, biochemical, and tissue culture techniques to support projects focused on viral infection, cancer, and inflammation. Work will require proficiency in basic cell biological laboratory techniques, including gene expression and knockdown, sterile mammalian cell culture, flow cytometry, PCR, immunoblot, ELISAs, immunofluorescence staining, and immunohistochemistry. These assays will also require the incumbent to utilize equipment and software to perform light and fluorescence microscopy, image acquisition, image analysis, and statistical analysis methods. Also participates in animal studies including surgeries and injections and assist in maintaining a mouse colony.
Theoretical and practical knowledge of virology, including relevant laboratory techniques to evaluate viral infection and virus-host cell interactions.
Theoretical and practical knowledge of biology, molecular biology, and biochemistry including relevant laboratory techniques acquired through a combination of extensive study and experience.
Strong research laboratory experience with in vitro techniques including cell culture and molecular biology methods.
Proven experience with assays to determine protein expression, including flow cytometry, immunoblotting, and immunostaining.
Strong experience with assays to determine gene expression, including RT-PCR.
Demonstrated ability with animal (mouse) techniques, specifically surgeries, injections, and cancer models.
Demonstrated experience in analysis and management of complex data. Excellent communication and record keeping skills.
Experience executing and overseeing research projects. Ability to train other investigators in the lab and have experience in this training. Ability to set priorities and to organize a varied workload to meet deadlines despite frequent interruptions.
Experience with Cal-OSHA & UCSD EH&S safety requirements including MSDS's, hazard communication & Injury and Illness Prevention Program; laboratory safety precautions including appropriate clothing and shoes, use of protective items such as gloves and goggles as well as basic first aid procedures.
Bachelor's Degree in Virology, Biology, Immunology, Genetics, Cancer, or related area of Biomedical Sciences.
- Employment is subject to a criminal background check.
- Willing to work with BSL3 viruses, hazardous materials (biological and chemical), and animals.
- Ability to work various hours and weekends as experimental protocols demand.
Apply on company website