UCSD Department of Ophthalmology is one of the most innovative and productive academic departments in the country, offering challenging career opportunities in the diverse areas of cutting-edge research, and state-of-the-art clinical services. The department is committed to offering a dynamic learning environment and growing opportunities to its talented and dedicated employees.
Perform duties as a research assistant in a laboratory studying molecular mechanisms of eye development and disease, human pluripotent stem cells in vitro to model retinal development and disease and uses gene-editing methods to genetically modify human stem cells for studies on gene function. Responsibilities will be to support all aspects of the lab including stem cell cultures, maintaining and differentiating stem cells, immunocytochemistry, PCR, support for molecular biology related to gene-editing of human pluripotent stem cells. Incumbent will assist with lab organization and general support.
Theoretical knowledge such as Molecular Biology, Structural Biology, General Chemistry or Genetics, or equivalent combination of education and experience including lab experience. Examples include PCR, plasmid cloning, sequencing.
Experience with cell cultures, preferably pluriopotent stem cells.
Experience performing molecular biology techniques including genetic manipulation, transfection of cells in tissue culture, molecular techniques to evaluate expression, localization and function of targeted molecules.
Experience in maintaining a well-organized lab notebook and thorough records. Basic math skills and ability to handle laboratory tasks.
Excellent interpersonal skills with strong, demonstrated ability to communicate orally and in writing. Candidate must possess tact, poise, diplomacy, confidentiality and flexibility in a lab environment that interacts with a diverse group of research and technical personnel and other laboratories.
Solid computer skills including the ability to create and maintain spreadsheets/databases, perform standard computer tasks, learn new programs/applications with minimal direction.
Knowledge of basic lab safety requirements/procedures; EH&S regulations and methods for handling, clean-up, and waste disposal for biohazardous materials.
Demonstrated experience with general lab maintenance including cleaning, stocking supplies.
Oral and written communication skills for presenting results to senior staff, writing reports, reviews and summaries.
- Employment is subject to a criminal background check.
- Must be willing to work with pluripotent stem cells (including NIH approved lines of embryonic origin).
- Must be available to work some weekends and evenings to complete experiments as needed.
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